Structural Analysis of the Glycine-rich, Arginine-rich Histone

A large scale procedure for isolation of the glycine-rich, arginine-rich histone and the arginine-rich, lysine-rich histone in a highly purified form.

1. A procedure is presented for large scale fractionation of arginine-rich histones by exclusion chromatography. With this method, 2 to 3.5 g of histone Fractions 2a and 3 were fractionated. The two fractions contained the same histone components, but they differed in the amounts of each component. 2. In histone Fraction 2a, the glycine-rich, arginine-rich (GAR) histone accounted for approximat...

Structure of the glycine-rich, arginine-rich histone of the Novikoff hepatoma.

woman-defined identity: analysis of selected poems of adrienne rich

the current thesis is composed in five chapters in the following fashion: chapter two encompasses the applied framework of the project in details; the methodology of carl gustav jung to explain the process of individuation, the major archetypes and their attributes and his techniques to assess the mind’s strata are all explained. moreover, the austrian psychoanalysts, heinz kohut’s models of a...

Structural analysis of the glycine-rich, arginine-rich histone. 3. Sequence of the amino-terminal half of the molecule containing the modified lysine residues and the total sequence.

1. The amino acid sequence of the amino-terminal portion of the glycine-rich, arginine-rich histone (Residues 1 to 49) has been determined from studies on the tryptic, chymotryptic, and thermolysin peptides. This sequence overlaps with that of the carboxylterminal portion (Residues 50 to 102) defined previously to give the following sequence: acetylserGly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys...

Structural analysis of the glycine-rich, arginine-rich histone. II. Sequence of the half of the molecule containing the aromatic amino acids.

1. The glycine-rich, arginine-rich histone from calf thymus was hydrolyzed with chymotrypsin; the peptides produced were fractionated on Sephadex G-25. The peptides containing the aromatic amino acids were purified by rechromatography on Sephadex G-25 and Aminex A-4 columns and by peptide mapping. 2. The six aromatic amino acids of the glycine-rich, arginine-rich histone were accounted for in e...